Bp 404 en

From DCEwiki
Jump to navigation Jump to search

Optically controlled and sensed micromanipulation - basic setup

Author: Jakub Tomášek

Bakalářské práce 2013

Download thesis in PDF

404 fig.gif

This thesis investigates two topics related to the use of optics in dielectrophoresis. First, it focuses on optically induced dielectrophoresis, which is a physical phenomenon enabling contactless manipulation with microparticles by projecting specialized light patterns onto a substrate. The main outcomes of the thesis are the design and the realization of a laboratory setup. In addition, numerous experiments were carried out to confirm the functionality. In the experiments, negative dielectrophoretic force induced by the image patterns was ob-served. Second, the thesis examines the applicability of a cheap lensless CMOS image sensor as a minimalistic solution for an optical feedback for fluidic micromanipulation platforms. The sensor can detect objects as small as 5µm, for example yeast cells. Also, a precise feedback manipulation with 50-micron polystyrene beads is demonstrated. It is shown that the accuracy provided by this cheap lensless sensor is comparable to that provided by a bulky combination of a standard microscope and an off-the-shelf camera. The experiments in both parts of the thesis are accompanied by numerical simulations.

Bp 2013 tomasek jakub.pdf